Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media

Moniruzzaman, M. and Nao, Y. and Bhattacharjee, S. and Ono, T. (2014) Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media. Applied Mechanics and Materials, 625. pp. 333-336.

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Abstract

Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) C2mimPF6 (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions. © 2014 Trans Tech Publications, Switzerland.

Item Type: Article
Impact Factor: cited By 6
Uncontrolled Keywords: Biological materials; Catalyst activity; Emulsification; Emulsions; Enzyme activity; Enzyme immobilization; Enzymes; Liquids; Polyesters; Polyethylene glycols; Polymers; Polyols; Scanning electron microscopy, Biological functions; Hexafluorophosphates; Identical conditions; Initial activity; Poly lactide; Polymer based composite; Storage stability; Water in oil emulsions, Ionic liquids
Depositing User: Ms Sharifah Fahimah Saiyed Yeop
Date Deposited: 29 Mar 2022 03:38
Last Modified: 29 Mar 2022 03:38
URI: http://scholars.utp.edu.my/id/eprint/31858

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